WebJun 4, 2024 · Phosphorylation of the effector kinase CHK1 at S345 and S317 by ATR enhances its kinase activity, resulting in dissociation from chromatin, phosphorylation of CDC25A-C phosphatases, which prevent the progression of the cell cycle [ 11, 12, 13 ]. WebChk1 is phosphorylated by ATR on serines 317 and 345 in a DNA damage-dependent manner. These phosphorylations are critical ... Antibodies used in this study included phosphospecific rabbit polyclonal antibodies against Chk1 S317, Chk1 S345, Nbs1 S343, and Smc1 S966 (all from Cell Signaling), total TopBP1 (Abcam), Claspin (Bethyl and …
Phospho-CHK1 (Ser345) Antibody (PA5-34625)
WebIn siGFP-transfected cells, p-Chk1 (S345) and p- Cdk2 (T14, Y15) were detected, indicating that HU treatment induced cell cycle arrest ( Figure 4C). p-Chk1 and p-Cdk2 levels were lower in HU ... WebSep 5, 2013 · The Chk1/2 inhibitor drug (AZD7762) was obtained from AstraZeneca. The drug powder was dissolved in 100% dimethyl sulfoxide (DMSO) to a stock concentration of 10 mmol/L and stored in aliquots. Intermediate drug concentration of 500 μmol/L was prepared from this stock and aliquoted. the pentitude bible
HU induces hMYH and hRad9 expression. (A) Reverse...
WebJul 17, 2024 · These results also suggest that the ATR kinase is required for the full CHK1 activation in vivo as the CHK1 variants, S317A and S345A (ATR-phosphorylation sites), are dominant negative even in... WebMar 14, 2024 · Alzheimer’s disease (AD) is the most common neurodegenerative disease with limited therapeutic strategies. Cell cycle checkpoint protein kinase 1 (Chk1) is a Ser/Thr protein kinase which is activated in response to DNA damage, the latter which is an early event in AD. However, whether DNA damage-induced Chk1 activation participates in the … WebWestern blot - Phospho-Chk1-S345 Rabbit pAb (AP0578) Western blot analysis of extracts of various cell lines, using Phospho-Chk1-S345 antibody (AP0578). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Western blot - Phospho-Chk1-S345 Rabbit pAb (AP0578) sia orthodontic dental specialist clinic